Figure 2.

Human Rrn3 crossreacts with anti-yRrn3 antibodies. E. coli-expressed 6His fusions of yeast and hRrn3 are shown. To compensate for differences in expression levels of the recombinant proteins, the volume of each lysate loaded was adjusted to permit equal amounts of yeast and hRrn3 to be compared. Nineteen microliters of yeast, 2 μl of human, or 19 μl of vector control lysate were resolved by SDS/PAGE and probed with monoclonal anti-6His antibodies (lanes 1–3) or with anti-yRrn3 antiserum (lanes 4–6). Positions of protein molecular weight markers are indicated.