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. 2000 Apr 11;97(9):4724–4729. doi: 10.1073/pnas.080063997

Figure 3.

Figure 3

Human RRN3 complements the lethal phenotype of an rrn3− mutant. (A) rrn3− strain RLY303 was transformed with a 2-μm plasmid construct expressing polyoma-tagged yeast RRN3 (PyWT), human RRN3 (PyHu), or empty expression vector (PyVect). Two single colonies from each transformation were streaked on glucose (Glu) to assay rrn3 complementation or on galactose (Gal) as a positive control. Plates were incubated at the temperatures indicated for 3 days (30°C, 37°C) or 5 days (25°C) and photographed. (B) Effect of yeast or human RRN3 expression on cell growth. Five-fold serial dilutions of yeast (PyWT) or human (PyHu) RRN3 transformants were spotted onto glucose. Plates were incubated at 25°C, 30°C, or 37°C as described for A.