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. 2000 Apr 11;97(9):4748–4753. doi: 10.1073/pnas.080072497

Figure 3.

Figure 3

Effect of missense mutations on expression of HIF-1α(1–391/512–826). (A) Immunoblot assay of nuclear extracts from 293 cells transfected with expression vectors encoding HIF-1α(1–391/512–826) (lanes 1–2), HIF-1α(1–391/512–826/C520 M) (lanes 3–4), or HIF-1α(1–391/521–826) (lanes 5–6). (B) Analysis of nuclear extracts from cells transfected with empty vector (EV; lanes 1–2) or HIF-1α constructs consisting of residues 1–826 (lanes 3–4) or 1–391/512–826 that was either wild type (lanes 5–6) or contained S515A/S517A (lanes 7–8) or Y519F (lanes 9–10) missense mutations. (C) Analysis of nuclear extracts from cells transfected with HIF-1α constructs consisting of residues 1–391/521–826 (lanes 1–2), 1–391/517–826 (lanes 3–4), or 1–391/512–826 that was either wild type (lanes 5–6) or contained P513G/P516G (lanes 7–8) or E512G/E518G (lanes 9–10) missense mutations. (D) Summary of the data. The amino acid (AA) sequence of HIF-1α residues 512–521 and the effect of missense mutations and deletions (−) on O2-regulated expression are shown.