Figure 5.

Effect of deletion or missense mutations on HIF-1α-mediated reporter gene transcription. Hep3B cells were transfected with 5 μg of pSVβgal, 10 μg of p2.1 (containing a hypoxia response element upstream of an SV40 promoter-luciferase reporter gene), and 125 ng of expression vectors encoding either no protein (EV), full-length HIF-1α (FL), full-length HIF-1α with S551G/T552A missense mutations (MIS), or HIF-1α(1–391/521–826) (DEL). Transfected cells were incubated under 20% O₂ for 24 h. The luciferase:β-gal activity ratio was determined and normalized to the value obtained from cells transfected with empty vector (EV) to obtain the relative luciferase activity. Results shown represent the mean and standard error for three plates of transfected cells.