Figure 3.

(A) FACS analysis of PerC isolated from irradiated μ^−/− recipients 2 months after cotransfer of 2 × 10⁶ FACS-purified CD4⁺ T cells from wild-type (CD4⁺ C57 μ^+/+) or μ^−/− mice (CD4⁺ μ^−/−), 5 × 10⁶ PerC cells from C57/Igh^a and 3 × 10⁶ bone marrow cells from C57BL/6 (Igh^b) mice. Contour plots (5%) are shown. The relative frequency of total live CD19⁺, CD3⁻, CD4⁻, CD8⁻, and F4/80⁻ and IgD^hi IgM^lo follicular B-2 cells and IgD^lo IgM^hi CD5⁺ and CD43⁺ B-1 cells is indicated. (B) Summary of data from three independent transfer experiments with five groups (n = 6–10 per group) of recipient μ^−/− mice that had received PerC (Igh^a) and bone marrow (Igh^b) together with FACS-purified CD4⁺ or CD8⁺ T cells from either wild-type C57BL/6 or μ^−/− mice, or no T cells. Total numbers (± SE) of the indicated B cell subpopulations in the peritoneal cavity and spleen are shown, calculated from the relative cell numbers obtained by FACS and total live cell counts obtained by counting acridine orange/ethidium bromide-stained cells. (C) Serum levels of PerC donor (B-1)-derived IgM-a and bone marrow-derived IgM-b (means ± SE) in the five groups of recipient mice determined by ELISA.