Table 1.

The effect of Lsh deletion on splenic subpopulations

	Control	−/−
0	Total spleen, %	Total spleen, %
CD3+	30.4  ± 12	40.5  ± 18 (133%)*
B220+ IgM+	43.1  ± 11	20.6  ± 7.8 (48% P < 0.0001)
CD4+	15.5  ± 3.5	16.6  ± 10.2 (107%)
CD8+	7.7  ± 2.2	14.9  ± 7.3 (193% P < 0.01)
Mac1+	4.1  ± 0.9	6.8  ± 1.8
Mac1+GR1+	5.8  ± 1.6	20.7  ± 8.3

Fetal liver cell suspensions of Lsh−/− and +/+ embryos were injected into Rag-2−/− recipients, and spleens were harvested after 5 wk. Splenocytes were analyzed by FACS for expression of T cell surface markers CD3, CD4, and CD8, or B cell surface markers B220 and IgM. Values represent percentage positive FACS stain of total spleen population and SD. Average numbers summarize four individual experiments with Lsh−/− (n = 11) and control (n = 19). Percentage difference in comparison with control and statistically relevant P values are indicated in parentheses. 

^*Indicates percentage change in comparison to control value that is set as 100%.