Table 1.

Oligonucleotides used for detection of ILT, LAIR, and KIR genes by PCR and determination of polymorphic sites by sequence analysis

	Sequence	Specificity
1	5′ GCCACAATCACTCATCAGAGTA 3′	ILT1 specific fwd
2	5′ GTATCGCTGTTACTATGGTAGCG 3′	ILT2 specific fwd
3	5′ ATGATCCCCACCTTCACGGCT 3′	ILT3 specific fwd
4	5′ CAGCTTCCATGCCTTCTGGG 3′	ILT3 specific rev
5	5′ TCAGTATTACAGCCGCGCTCGG 3′	ILT4 specific fwd
6	5′ GGTGCTATGGTTATGACTCGCGCG 3′	ILT6 specific fwd
7	5′ ATGACCGCCGCCCTCACAGCCT 3′	ILT9 specific fwd
8	5′ GCAGAGCAGGGCATCATGGTGT 3′	ILT10 specific fwd
9	5′ AGCCTCCGAGTGTCCACACTG 3′	LIR6 specific fwd
10	5′ TTGGCAGACAGTCCAGATAACATC 3′	LIR6 specific rev
11	5′ TGTCGTGGCCCGCGGAGGC 3′	LIR8 specific fwd
12	5′ TGACTGACACAGCAGGGTCACG 3′	ILT redundant rev
13	5′ CGTGACCCTGCTGTGTCAGTCA 3′	ILT4 sequencing primer
14	5′ CGAAGCCATGAGTTGCACACTG 3′	Marker 204 fwd
15	5′ CAACCACAGCATCTGTAGGCTCC 3′	Marker 204 rev
16	5′ GGGGAGCCATGTGACTTTCGTG 3′	LAIR fwd
17	5′ GTCACTCTGCTCAGACCATTTAG 3′	LAIR rev
18	5′ TGATTGGGACCTCAGTGGTCA 3′	KIR exon 7 redundant fwd
19	5′ CCCAACRCAYRCCATGMTGA 3′	KIR exon 1 redundant rev

The KIR oligonucleotides were used for linking adjoining genes on haplotype 1. KIR genes were detected by using primer sets as described (16).