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LTC maintain a population of DC progenitors. At day 0, size-sorted small cells were transferred onto an irradiated, spleen-derived stromal monolayer. A selected colony derived from transferred cell(s) was photographed at 8 days (a), 13 days (b), 16 days (c), 19 days (d), and 26 days (e) after establishment (×200). By 19 days (f), no cell growth had occurred on the stromal monolayer of the control flask supplemented with medium only (×200).
