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. 2000 Apr 11;97(9):4790–4795. doi: 10.1073/pnas.070061297

Figure 1.

Figure 1

Diagram of the human genomic MLL. The top map represents the BamHI (B) DNA gene segments with the size (kb) indicated above (22–24). The bottom map represents an enlargement of the 8.3-kb BamHI MLL BCR, which is involved in all MLL chromosome translocations. The entire MLL gene was analyzed for in vivo bioflavonoid- and VP16-induced cleavage sites, centromeric (cen) to telomeric (tel), by use of a series of MLL DNA probes from exons 1 to 34 and genomic fragments corresponding to each BamHI fragment (22–24); these include three centromeric DNA probes (gray boxes) amplified from the cosmid COS20 using PCR at positions 18,652–19,825, 19,936–20,542, and 32,119–32,442 (24). The 7.0- and 1.3-kb cleavage fragments (below the map) representing the BCR were identified with the 0.74-kb cDNA probe (exons 5–11, indicated above map line). The black arrows above both maps represent the colocalizing bioflavonoid- and VP16-induced in vivo topo II cleavage site between nucleotides 6,800 and 7,000 and the DNase I HS site mapped previously (21).