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. 2000 Apr 11;97(9):4790–4795. doi: 10.1073/pnas.070061297

Figure 4.

Figure 4

Southern blots were hybridized with the 0.74-kb MLL BCR cDNA probe. (a) MLL cleavage induced by 25 μM or 50 μM quercetin and VP16 for 6 h can be reversed using two different methods: A, heat treatment of cells for 15 min at 60°C, immediately after the 6 h drug incubation; or B, an additional incubation of the drug-treated cells in drug-free media for 2 h at 37°C. (b) MLL BCR DNA cleavage induced by genistein and VP16 using an in nuclei topo II assay. Isolated BV173 nuclei were incubated with 2 units or 4 units of purified human topo II and 50 μM VP16 or genistein was added for 5 min at 30°C.