Table 1.

Induction of CTL against hTRT in HLA-A2.1 transgenic mice

Group	hTRT peptide	Helper peptide	No. of responders	Percent lysis
1	540ILAKFLHWL548	−	10 /15 (66%)	(35, 21, 34, 42, 56, 21, 12, 35, 42, 16)
2	"	+	8 /10 (80%)	(45, 56, 62, 64, 65, 45, 65, 45)
3	865RLVDDFLLV873	−	3 /15 (20%)	(25, 12, 15)
4	"	+	7 /10 (70%)	(25, 32, 35, 12, 16, 18, 21)

HHD mice were immunized by a s.c. injection of 100 μg of hTRT peptide emulsified in incomplete Freunds' adjuvant. In Groups 2 and 4, the hTRT peptide was administered together with 140 μg of the helper peptide TPPAYRPPNAPIL (25). Values of cytotoxicity refer to individual responder mice. Spleen-derived CTL were harvested 7 days after immunization and then cultured for 6 days with the homologous hTRT peptide. Values refer to maximal cytotoxicity at an effector-to-target ratio of 60∶1.