Figure 2.

In vitro assay for endostatin activity. (A) Inhibition of bFGF (1 ng/ml) stimulated HUVEC proliferation by increasing amounts of purified, ADV.mEnd-expressed endostatin (hatched columns) and in the absence of endostatin (positive control; open column). HUVEC proliferation without bFGF (negative control; filled column). (B) Same assay as in A. Shown is a 2-fold dilution of conditioned supernatant of JC cells transduced with ADV.mEnd or ADV.β-Gal at different moi (in particles). ADV.mEnd: 1 × 10⁴ (○), 5 × 10⁴ (□), and 1 × 10⁵ (▵). ADV.β-Gal: 1 × 10⁴ (●) and 5 × 10⁴ (■). pos., Complete medium with bFGF; neg., complete medium without bFGF. Data are shown as the mean of six wells. Bars indicate ± SD.