Table 1. Transformation frequencies.
Construct | % regeneration | % PCR-positive regenerants | % Transgenosis |
pK-I | 61 | 66 | 40 |
pK-BI | 24 | 41 | 10 |
pK-YBI | 47 | 37 | 17 |
pP-I | 73 | 71 | 52 |
pP-BI | 29 | 38 | 11 |
pP-YBI | 64 | 57 | 36 |
The % of leaf discs giving at least 1 regenerant after 8 weeks on kanamycin is shown in the second column. The % of PCR-positive shoots containing the transgene are shown in the third column. The % transgenosis (fourth column) indicates the % of leaf disks giving at least 1 PCR-positive regenerant.