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. Author manuscript; available in PMC: 2007 Mar 23.
Published in final edited form as: Gene Ther. 2004 Sep;11(18):1399–1407. doi: 10.1038/sj.gt.3302307

Figure 3. Levels of prostate-specific expression induced by the PFLPS plasmid vectors are greater than that induced by the non-specific, Tet-regulated control.

Figure 3

LNCaP and U343MG cells were seeded in 24-well plates and co-transfected with either Tet plasmids (pUHD 15-1 plus pRAd2T.GFP.B), ARR2PB plasmid (pRAd22Pb.GFP plus empty vector), PFLPS plasmids (pLAd(T2Pb.tTA.S)r plus pRAd2T2Pb.GFP.B) or empty vector using SuperFect reagent according to manufacturer’s instructions in the presence of 30μM DHT. 3 days post-transfection, cell lysates were assayed for GFP fluorescence on FLUOstar fluorescence plate reader. RFU: relative fluorescence units. Numbers above LNCaP data bars indicate fold-induction relative to transfection with ARR2PB plasmid (set as 1.0x).