Figure 4. Levels of prostate-specific expression induced by the Ad/GFPPFLPS vector is greater than that induced by the non-specific, Tet-regulated control.
LNCaP, 22RV1, and U251MG cells were seeded in 24-well plates and infected with Ad vectors at MOI 50 in the presence or absence of 30nM DHT. 2 days post-infection, cell lysates were assayed for GFP fluorescence. Infection with HBS served as mock infection control. (a) GFP fluorescence as measured in relative fluorescence units (RFU). (b) GFP fluorescence calculated as percent GFP expression, setting GFP fluorescence as a result of Ad/GFPTET infection at 100% for each cell line. *p < 0.05 compared to mock. **p < 0.01 compared to mock.