Figure 5. Transduction with Ad/GFPPFLPS vector induces dose-dependent GFP expression in LNCaP cells while retaining prostate-specificity in U251MG cells.
LNCaP and U251MG cells were seeded in 96-well plates and infected with Ad vectors at MOI 0, 10, 100, or 1000 in the presence of 30nM DHT. 2 days post-infection, cell lysates were assayed for GFP fluorescence. Infection with Ad/C.LacZ served as vector infection control. GFP fluorescence of (a) LNCaP cells and (b) U251MG cells. *p < 0.05 compared to Ad/C.LacZ. **p < 0.01 compared to Ad/C.LacZ.