Table 1.
Effect of Lf on bacterial growth in ISB culture, measured by lag time and maximum absorbance.
Lag time (h) | Maximum absorbance | |||||
Bacteria | Mean | Min-max | SD | Mean | Min-max | SD |
Escherichia coli (n = 5) | ||||||
Control | 3.8 | 3.0–4.0 | 0.45 | 0.91 | 0.70–1.05 | 0.15 |
0.67 mg/ml Lf | 6.6 | 5–9 | 1.52 | 0.67 * | 0.57–0.73 | 0.06 |
1.67 mg/ml Lf | 9.0 | 5–15 | 4.1 | 0.52 * | 0.25–0.70 | 0.18 |
2.67 mg/ml Lf | 10.6 | 6.0–20.0 | 6.0 | 0.42 * | 0.11–0.68 | 0.24 |
Staphylococcus aureus (n = 5) | ||||||
Control | 4.8 | 4.0–5.0 | 0.45 | 0.60 | 0.49–0.65 | 0.07 |
0.67 mg/ml Lf | 7.2 | 5.0–11 | 2.4 | 0.55 | 0.42–0.69 | 0.11 |
1.67 mg/ml Lf | 8.4 * | 6.0–12 | 2.2 | 0.52 | 0.40–0.66 | 0.10 |
2.67 mg/ml Lf | 8.4 * | 6.0–12 | 2.2 | 0.49 * | 0.40–0.64 | 0.10 |
Coagulase-negative staphylococci (n = 5) | ||||||
Control | 6.4 | 5.0–10 | 2.2 | 0.56 | 0.53–0.60 | 0.03 |
0.67 mg/ml Lf | 12.2 | 6.0–20 | 7.2 | 0.36 | 0.10–0.56 | 0.23 |
1.67 mg/ml Lf | 12.2 | 6.0–20 | 2.2 | 0.34 | 0.11–0.52 | 0.21 |
2.67 mg/ml Lf | 12.4 | 7.0–20 | 7.0 | 0.34 | 0.10–0.55 | 0.21 |
Lag time = time from the beginning of incubation until the time-point when the absorbance began to increase; maximum absorbance = highest absorbance value measured during the 20-h incubation period.
Statistically significant difference when compared with negative control: * p ≤ 0.05