FIGURE 6.

Immunostaining and FRI of focal adhesion proteins. REF52 cells transfected with YFP-paxillin were fixed and immunostained with primary antibody against zyxin, followed by Cy5-conjugated secondary antibodies. Actin filaments were visualized with phalloidin-TRITC. Cells on 58-nm and 108-nm RGD-nanopatterns were observed at 3 h and 24 h after plating. The rows present the images with paxillin in red, zyxin in blue, and actin in green. The last row shows the ratio between paxillin and zyxin intensities. FRI are presented in a spectrum scale as indicated in the lookup table.