AFM height images and fluorescent images (labeled with a star) of supported lipid bilayers formed from DOPC/GalCer/chol (top row), POPC/GalCer/chol (middle row), and DLPC/GalCer/chol (bottom row) at various cholesterol concentrations. For SLBs containing 0 mol % chol, the mean values of domain height by section analysis were determined to be 0.85 ± 0.12 nm, 0.60 ± 0.07 nm, and 0.88 ± 0.11 nm for DOPC/GalCer, POPC/GalCer, and DLPC/GalCer SLBs, respectively. The mean height value of all other domains was <1 nm. Scale bars are 10 μm unless otherwise specified.