With the variation of parameter , a–c, plot in the versus plane of curve (thick line) and curve (thin line), the cross point of the two curves gives the steady state of the system. (d–f) The activity of and promoter change as a function of CI or Cro total concentration. The thick solid line represents , the thick shaded line represents , and the thin solid line represents . In these subfigures, the value of is −6.3 kcal/mol in a and d; −3 kcal/mol in b and e, and −8 kcal/mol in c and f.