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. 1992 Nov;58(11):3762–3766. doi: 10.1128/aem.58.11.3762-3766.1992

Purification and partial characterization of two feruloyl esterases from the anaerobic fungus Neocallimastix strain MC-2.

W S Borneman 1, L G Ljungdahl 1, R D Hartley 1, D E Akin 1
PMCID: PMC183171  PMID: 1482195

Abstract

Two extracellular feruloyl esterases (FAE-I and FAE-II) produced by the anaerobic fungus Neocallimastix strain MC-2 which cleave ferulic acid from O-(5-O-[(E)-feruloyl]-alpha-L- arabinofuranosyl)-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-D-xylopyranose (FAXX) were purified. The molecular masses of FAE-I and FAE-II were 69 and 24 kDa, respectively, under both denaturing and nondenaturing conditions. Apparent Km and maximum rate of hydrolysis with FAXX were 31.9 microM and 2.9 mumol min-1 mg-1 for FAE-I and 9.6 microM and 11.4 mumol min-1 mg-1 for FAE-II. FAE-II was specific for FAXX, but FAE-I hydrolyzed FAXX and PAXX, the equivalent p-coumaroyl ester, at a maximum rate of metabolism ratio of 3:1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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