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. 2007 Jan 26;92(8):2926–2943. doi: 10.1529/biophysj.106.101428

TABLE 5.

Simulated intensity distributions and best fit results for Case III

Simulated distribution fit with
Simulation Simulation value* MD histograms RA histograms
1MD Inline graphic 1500 1478 1721
Inline graphic 1500 1515 1059
N 2995 2994
Inline graphic 2 214
Inline graphic 0.34 0.74
3MD Inline graphic 1500 1291 1209
Inline graphic 1500 1595 598
N 2992 2992
Inline graphic 6 1185
Inline graphic 0.55 0.89
4MD Inline graphic 1500 1385 738
Inline graphic 1500 1482 736
N 126 2993
Inline graphic 2993 1519
Inline graphic 0.47 0.73
6MD Inline graphic 3000 2402 535
N 2988 2973
Inline graphic 586 2438
Inline graphic 0.95 0.44
7MD Inline graphic 200 202 329
Inline graphic 1200 1165 1354
Inline graphic 1200 1278 795
Inline graphic 400 287 299
N 2992 2990
Inline graphic 60 213
Inline graphic 0.52 1.22
8MD Inline graphic 200 144 824
Inline graphic 1200 1516 650
Inline graphic 1200 753 773
Inline graphic 400 579 66
N 3000 3000
Inline graphic 8 687
Inline graphic 0.39 0.57

Simulated intensity distributions and best-fit results for examples in Case III, where Inline graphic but there is no significant difference in Inline graphic between the MD and RA fits. Each set of coefficients was used to generate intensity distributions using both the MD and RA process, and both resultant distributions were then fit using both the MD and RA basis histograms. The list of coefficients includes only those that had nonzero values in the simulated intensity distribution, and their sum is 3000 for all simulations. N′ is the sum of all best coefficients. Inline graphic is the sum of the unlisted coefficients, that is, Inline graphic is the sum of those best-fit coefficients that would have been zero for a perfect fit. In some instances, the sum of the coefficients listed exceeds N′ due to rounding. Eight basis histograms were used for all fits listed in this table, and α = 0.001 unless otherwise stated.

*

Values of Inline graphic used to generate the simulated intensity distribution.

Ten RA basis histograms were used in this fit.

Fourteen RA basis histograms were used in this fit.