Figure 3.
Deglycosylation analysis of BBP-A. The BBP-A produced in insect cells was treated with Endo Hf glycosidase (Bi+) and analysed with SDS-PAGE. Untreated control sample (Bi) and BBP-A produced in bacteria (Bb) were also analysed. For comparison, the same analysis was performed for AVD isolated from chicken (Ac+). Chicken AVD control sample (Ac) as well as AVD produced in bacteria (Ab) [30] are also shown. The molecular weight markers are shown on both sides of the gel (14.4 and 21.5 kDa).