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Calcium-permeant nature of the nonselective cation channel activated by gp120. Currents were recorded by using a pipette solution containing 120 mM Cs-aspartate and 25 mM CsCl, and extracellular bath containing 100 mM CaCl2 and no other cations. The membrane was voltage-clamped at the calculated ECl (−50 mV), and NPPB (10 μM) was added to the bath. An inward calcium current was elicited by gp120 from JRFL (A) and IIIB (B) but not by MIP-1β (C) or SDF-1α (D).
