. Author manuscript; available in PMC: 2007 Mar 25.

Published in final edited form as: Arch Biochem Biophys. 2006 Oct 30;458(2):121–127. doi: 10.1016/j.abb.2006.10.005

Table 2.

Purification of quail liver binding protein (qlLBP).

Steps	Column^*	Total Protein (μg/μl)	A₅₃₇/₂₈₀	Lutein (% of total carotenoids)	Purification
1	Extract	1.4	0.02	54	1
2	A (unbound)	0.84	0.039	63	2
3	Q (unbound)	0.23	0.32	72	16
4	S (bound)	0.08	0.62	78	31.0
5	Silica gel	0.008	0.73	91	36.5

A and Q are anion-exchange columns, and S is a cation-exchange column used for purification.