Fig. 2.
Antagonism of C/EBPα truncated isoforms mediated transactivation on the amelogenin promoter by Msx2. In the presence of 250 ng of p2207-luc reporter construct and 75 ng of pCMV-lacZ, various amounts (100, 200, and 400 ng) of the Msx2 expression plasmid were cotransfected into LS8 cells in the presence (+) or in the absence (−) of 200 ng of the expression plasmid encoding CR1/2/3/4, CR2/3/4, CR2/4, CR1/2, or CR2, respectively. The relative luciferase activity is the normalization of luciferase activity with β-galactosidase activity. The mean ± S.D. from at least three independent experiments is represented, and the basal level of p2207-luc was set as 1.