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. Author manuscript; available in PMC: 2008 Jan 5.
Published in final edited form as: J Mol Biol. 2006 Oct 4;365(1):50–65. doi: 10.1016/j.jmb.2006.09.08

Figure 2. FCS analysis of allophycocyanin and importin β in vitro and in vivo.

Figure 2

Autocorrelation functions for APC and importin β labeled with Alexa Fluor 647 were measured in vitro and inside live cells and normalized. Autocorrelation data for both APC and importin β in vitro were fitted with a single decay time τ = 300 μs. Autocorrelation data for APC in vivo was fitted with a decay time τ = 1000 μs. Autocorrelation data for importin β in vivo required multiple decay times for an adequate fit.