Figure 1. A. Cell viability assay.
Normal ONH astrocytes were treated with varying concentrations of HNE for 6 h. Treatment with HNE at concentrations of 50 μM and higher led to a greater than 50% reduction in cell viability over 6 h. The data is expressed as % of viable cells of the untreated control. Asterisks indicate significant difference (*P<0.05).
B. Time course of HNE (25 μM) treatment. Normal ONH astrocytes were treated with 25 μM HNE for different exposure times. Treatment with 25 μM HNE for 3 h led to a 10% reduction in cell viability and was used in future experiments. Asterisks indicate significant difference (*P<0.05).