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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Exp Eye Res. 2006 Dec 13;84(3):444–454. doi: 10.1016/j.exer.2006.10.020

Figure 4. Effects of HNE on transcription factor gene expression.

Figure 4

Normal astrocytes were treated for 1 h and 3 h with 25 μM HNE, then the HNE-containing media was removed and RNA was collected 6 h later. Relative amount of target mRNA in ONH astrocytes measured by quantitative RT-PCR as described in the Methods. Values represent mean ±SD of target mRNA level normalized to 18S RNA (internal control). All experiments were done in triplicate using three normal astrocytes cultures. Asterisk indicates significance of P <0.05.

A. Increased expression of Nrf2 using qRT-PCR. The relative expression of Nrf2 mRNA increased in response to both a 1 h and 3 h HNE treatment to 1.80 and 1.75 times higher than control levels, respectively.

B. Induction of cFOS expression. The relative expression of cFOS mRNA increased after 1 h and 3 h HNE. A smaller increase of cFOS mRNA occurred after 1 h HNE treatment to 1.86 times higher than control levels, and a great increase of cFOS mRNA to 34.96 times higher than control levels occurred after 3 h HNE treatment.

C. Expression of NFkB. The relative expression of NFkB did not change after treatment with HNE. All experiments were done in triplicate using three normal astrocytes cultures.