Skip to main content
letter
. 2007 Apr;17(4):470–481. doi: 10.1101/gr.6130907

Figure 4.

Figure 4.

The 8q24.1 breakpoint localizes to a 3304-bp region within the first intron of the RNF139 gene. (A) Schematic representation of the 8q24.13 region showing the positions of the 8.3, 8.4, 8.5, and 8.6 amplicons used in mapping experiments relative to an AT-rich repeat present between the 8.3 and 8.4 primer pairs. Relative positions of restriction fragments analyzed in Southern blot experiments are also shown. The breakpoint identified by nested PCR experiments is also indicated. (B) PCR-mediated mapping of 8q24.1 sequences was performed on total genomic DNA relative to DNA template from LMPC-dissected der(8) and der(22) chromosomes. Results from the 8.3, 8.4, 8.5, and 8.6 amplicons are shown. (C) Southern blot analysis of genomic DNA from normal control sample (“C”) and the t(8;22) carrier (“T”) digested with EcoRV, KpnI, and NdeI restriction enzymes. Amplicons 8.4 and 8.5 were radiolabeled and used as probes. Arrows indicate the position of expected restriction fragments.