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Agarose gel electrophoresis and Southern hybridization of plasmid DNA isolated from B. burgdorferi B31 erythromycin-resistant clones electroporated with plasmid pGK12. (a) Agarose gel electrophoresis. (b) Southern blot of agarose gel in a. Lane 1, pGK12 DNA purified from E. coli MM294; Lane 2, Plasmid DNA from wild-type B. burgdorferi B31; Lanes 3–5, Plasmid DNA of three different B. burgdorferi erythromycin-resistant electroporants containing pGK12.
