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. 2000 Apr 18;97(9):4850–4855. doi: 10.1073/pnas.080068797

Figure 4.

Figure 4

Detection of EGFP and egfp mRNA in B. burgdorferi B31 containing plasmid pMS1. (a) Immunodetection of EGFP. Lane 1, protein lysate of E. coli containing pMS1. Lane 2, protein lysate of wild-type B. burgdorferi B31. Lanes 3 and 4, B. burgdorferi isolates containing pMS1. (b) RT-PCR of egfp RNA. Lane 1, molecular weight standards. Lane 2, amplification of RNA from B. burgdorferi containing pMS1 without reverse transcriptase. Lane 3, amplification of RNA from B. burgdorferi containing pMS1 with reverse transcriptase. Lanes 4 and 5, the same as 2 and 3, but with cultures of B. burgdorferi shaken in the presence of oxygen. Lane 6, RT-PCR performed without RNA.