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. 2000 Apr 18;97(9):4850–4855. doi: 10.1073/pnas.080068797

Figure 5.

Figure 5

Competitive PCR of egfp and flaB DNAs in B. burgdorferi containing pMS1. All reactions except negative controls contained 0.5 ng of total DNA from B. burgdorferi cells with pMS1. In flaB (lanes 1–7) and egfp (lanes 8–14), the competitive amplifications were performed with primers specific to the flaB and egfp genes, respectively. The PCR reactions contained 1 pg of correspondent competitor (lanes 1 and 8), 300 fg (lanes 2 and 9), 100 fg (lanes 3 and 10), 33 fg (lanes 4 and 11), 10 fg (lanes 5 and 12), and 3.3 fg (lanes 6 and 13). Lanes 7 and 14 correspond to reactions carried out without DNA.