Figure 3.

Western blots of 40 known or predicted yeast transcription factors. Representative Western blot analysis of 40 known or candidate yeast TFs. N-terminally GST-tagged proteins were overexpressed in and purified from E. coli in high-throughput, as described in Methods. Five microliters out of 60 uL total of each purified protein were analyzed by Western blots using anti-GST antibody. Serial dilutions of recombinant GST (Sigma) were included for estimation of protein concentrations. Bands of the correct size or the positions of the expected size are indicated by a dot on the right side of the band.