. Author manuscript; available in PMC: 2008 Jan 19.

Published in final edited form as: J Mol Biol. 2006 Oct 3;365(3):835–855. doi: 10.1016/j.jmb.2006.09.083

Table 3.

Rate constants for duplex-unwinding and strand-annealing of RNA oligonucleotide substrates by Mss116p.

	k_unw (min⁻¹)	k_{ann[no ATP]} (x10⁹ M⁻¹ min⁻¹)	k_ann[ATP] (x10⁹ M⁻¹ min⁻¹)
5′ overhang	0.91 ± 0.07	4.7 ± 0.8	5.6 ± 1.7
3′ overhang	1.21 ± 0.31	5.6 ± 0.5	7.8 ± 2.0
Blunt	1.0 ± 0.2	6.2 ± 0.6	4.9 ± 1.1

Rate constants were calculated from at least two repeats of reactions shown in Figure 1. RNA substrates contained a 16-bp duplex with a 5′- or 3′-overhang or blunt ends (see Materials and Methods).