Table 3.
kunw (min−1) | kann[no ATP] (x109 M−1 min−1) | kann[ATP] (x109 M−1 min−1) | |
---|---|---|---|
5′ overhang | 0.91 ± 0.07 | 4.7 ± 0.8 | 5.6 ± 1.7 |
3′ overhang | 1.21 ± 0.31 | 5.6 ± 0.5 | 7.8 ± 2.0 |
Blunt | 1.0 ± 0.2 | 6.2 ± 0.6 | 4.9 ± 1.1 |
Rate constants were calculated from at least two repeats of reactions shown in Figure 1. RNA substrates contained a 16-bp duplex with a 5′- or 3′-overhang or blunt ends (see Materials and Methods).