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. Author manuscript; available in PMC: 2007 Mar 26.
Published in final edited form as: Clin Cancer Res. 2006 Nov 15;12(22):6687–6695. doi: 10.1158/1078-0432.CCR-06-0921

Figure 1.

Figure 1

(A) Expression of SYK mRNA in HCC cell lines HepG2 and Hep3B. SYK mRNA expression was determined by RT-PCR. An RT-negative control (−) was used to rule out false positives resulting from contaminated genomic DNA. mRNA for β2-microglobulin (B2MG) was also analyzed to verify the RNA integrity. A blank control (H2O) was included in each PCR experiment. PCR products and a molecular weight (MW) marker were run on an agarose gel following by ethidium bromide staining. Bands of 507 bp and 115 bp are expected for SYK and β2-microglobulin transcripts, respectively. At least two independent experiments were carried out. (B) Restoration of SYK mRNA by treatment with DNMT1 inhibitor. HepG2 was treated for 5 days with (+) or without (−) 2.0 μM 5-aza-dC. As a control, Hep3B cells were processed in parallel. Total RNA was harvested and RT-PCR amplified as detailed in (A).