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. 2000 Apr 11;97(9):4862–4866. doi: 10.1073/pnas.080079397

Figure 3.

Figure 3

The plasmid pHA16–3 contains viral–cellular junctions involving both AAVS1 and TNNT1 sequences. (A) A schematic representation of viral-cellular junctions in the plasmid pHA16-3 is shown. The plasmid pHA16-3 was isolated from a genomic library of latently infected HeLa cells (HA16C5) by selection for hybridization to an AAV probe. Subsequently, the viral–cellular junctions were sequenced. pHA16-3 contains a 14-kb insert with one junction between AAV2 (light gray) and AAVS1 (dark gray) and a second one between AAV (light gray) and TNNT1 (intermediate gray). At each junction, one nucleotide (white box) is common to both sequences. Underlined nucleotide numbers correspond to sequences at the recombination junctions (AAV2, accession no. AF043303; TNNT1, accession no. AJ011713; AAVS1, accession no. S51329). Nucleotide 1046 in TNNT1 corresponds to the cloning site. The DNA sequences between the junctions have not been determined (thin line). (B) DNA sequences at the AAV-TNNT1 junction are shown. The AAV RBS and TRS sites are indicated.