Figure 3.

Susceptibility of J and CHO cells expressing mNectin1δ to infection with HSV, PrV, and BHV-1. Micrographs showing J cells expressing mNectin1δ (A and D–F), hNectin1α (B), or J cells transfected with pcDNA3.1(−) (C), infected with indicated viruses. CHO cells transfected with (G and H) mNectin1δ, or (I) pcDNA3.1(−), infected with (G) PrV or (H) BHV-1. Infection was detected as β-gal (A–F), or by immunostaining (G–I). Differences in intensities between A and B compared with D–F are because of the fact that in A and B, the LacZ gene is expressed from the viral genome, and supposedly in higher copy number, whereas in D–F, the LacZ is expressed from a gene resident in the cell.