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Detection of Us9 expression in infected PK15 cells by Western blot analysis. Lysates were probed with an anti-Us9 monoclonal antibody, and reprobed with an anti-gI polyclonal antiserum. The latter provided a gel loading control. Percentages listed at bottom are quantitation of Us9 signal relative to gI signal, as described in Materials and Methods. The quantitation was an average of two gels, only one of which is shown. PRV161 harbors a deletion in the Us9 gene.
