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. 2000 Apr 18;97(9):4879–4884. doi: 10.1073/pnas.080078197

Figure 2.

Figure 2

Representative two-hybrid interactions. Diploids containing an ORF from the vaccinia virus activation domain array (Leu+) and a test vaccinia ORF in the DNA-binding domain vector (Trp+) were plated onto synthetic medium lacking leucine, tryptophan, and histidine and supplemented with 3 mM 3-amino-1,2,4-triazole. Each member of the vaccinia activation domain array is represented as four independent colonies, and two-hybrid positives are observed as growth of three or four diploid colonies over a background of nonreproducible single colonies. (A) I3L self-association. (B) A20R interaction with H5R, D5R, and D4R. (C) H5R interaction with G2R and B1R. (D) A ORF D interaction with A23R. (E) D7R interaction with A32L, lower cluster (arrow) is a false positive. (F) A12L interaction with A19L.