Table 1.
Two-hybrid interactions of vaccinia virus proteins
| BD | AD | ||
|---|---|---|---|
| DNA replication | |||
| A20R | Putative polymerase processivity factor | H5R | Late transcription factor 4 |
| A20R | Putative polymerase processivity factor | D5R | Putative DNA replication factor |
| A20R | Putative polymerase processivity factor | D4R | Uracil DNA glycosylase |
| F2L*† | dUTPase | F2L | dUTPase |
| F4L†‡ | Ribonucleotide reductase small subunit | I4L | Ribonucleotide reductase large subunit |
| F4L*† | Ribonucleotide reductase small subunit | F4L | Ribonucleotide reductase small subunit |
| I3L* | Single-stranded DNA-binding protein | I3L | Single-stranded DNA-binding protein |
| I4L*† | Ribonucleotide reductase large subunit | I4L | Ribonucleotide reductase large subunit |
| J2R*† | Thymidine kinase | J2R | Thymidine kinase |
| Transcription | |||
| A ORF D | Hypothetical 9.8-kDa protein | A23R | Intermediate transcription factor 3 large subunit |
| A1L | Late transcription factor 2 | G8R | Late transcription factor 1 |
| A23R† | Intermediate transcription factor 3 large subunit | A8R | Intermediate transcription factor 3 small subunit |
| A49R | Hypothetical 18.8-kDa protein | H5R | Late transcription factor 4 |
| D7R | RNA polymerase subunit RPO 18 | A32L | Putative DNA-packaging protein, morphogenesis |
| F8L‡ | Hypothetical 7.8-kDa protein | L4R | DNA binding, helicase stimulation, virion transcription |
| G2R†‡ | Transcription elongation factor | H5R | Late transcription factor 4 |
| G8R* | Late transcription factor 1 | G8R | Late transcription factor 1 |
| H5R | Late transcription factor 4 | B1R | Protein kinase 1 |
| Virion structure | |||
| A10L* | Major core protein P4A | A10L | Major core protein P4A |
| A12L‡ | Virion protein | A19L | Hypothetical 8.3-kDa protein |
| A14L | Phosphorylated membrane protein | A40R | Hypothetical 19.3-kDa NK cell receptor homolog |
| A32L | Putative DNA-packaging protein, morphogenesis | A11R | Hypothetical 36.1-kDa protein |
| A45R | Superoxide dismutase (Cu-Zn) homolog | A4L | Virion protein, between core and membrane |
| J1R | Virion protein | A45R | Superoxide dismutase-like (Cu-Zn) |
| J1R* | Virion protein | J1R | Virion protein |
| Virus–host interactions | |||
| A26L | Cowpox A-type inclusion protein homolog | A42R | Profilin homolog |
| E3L*† | Double-stranded RNA-binding protein, IFN resistance | E3L | Double-stranded RNA-binding protein, IFN resistance |
| K1L† | Host range factor | C10L | Hypothetical 38.5-kDa protein |
| E7R | Soluble, myristylated, nonessential | A39R | Semaphorin, cytokine induction |
| Unknown function | |||
| A21L | Hypothetical 13.6-kDa protein | A6L | Hypothetical 43.1-kDa protein |
| A22R* | Hypothetical 20.7-kDa protein | A22R | Hypothetical 20.7-kDa protein |
| A45R* | Superoxide dismutase (Cu-Zn) homolog | A45R | Superoxide dismutase (Cu-Zn) homolog |
| A51R* | Hypothetical 31.7-kDa nonessential protein | A51R | Hypothetical 31.7-kDa nonessential protein |
| E8R | Hypothetical 31.9-kDa protein | A51R | Hypothetical 31.7-kDa nonessential protein |
| F15L | Hypothetical 18.6-kDa protein | D9R | MutT family member |
| 12L | Hypothetical 35.8-kDa protein | A40R | Hypothetical 19.3-kDa NK cell receptor homolog |
| Unnamed‡ | Hypothetical 8.9-kDa protein (ORF position R-111049-111279) | E10R | Hypothetical 10.8-kDa protein |
*Self-interacting proteins.
†Previously observed interactions.
‡Reciprocal interactions observed in both directions.