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. 2000 Apr 11;97(9):4885–4890. doi: 10.1073/pnas.060030097

Figure 2.

Figure 2

Swarming motility in P. aeruginosa PAO1. The ppk mutant is completely deficient in swarming motility which could be restored to the WT level by providing a plasmid bearing the ppk gene. Cells were inoculated onto swarm plates (Difco nutrient broth plus 0.5% Difco bacto-agar supplemented with 0.5% glucose) from an overnight swim plate with a toothpick and photographed after a 24-h incubation at 30°C. Strains: WT, PAO1; Δppk, PAOM5; ΔfliC, MS159; ΔfliD, PAO-D; ΔfliF, PA14-fliF; WT (CV), PAO1 (pMMB66HE); Δppk (CV), PAOM5 (pMMB66HE); and Δppk (PPK+++), PAOM5 (pHEPAK11). CV represents the control vector pMMB66HE and PPK+++ the PPK-overproducing plasmid pHEPAK11.