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. 2000 Apr 18;97(9):4914–4919. doi: 10.1073/pnas.090092797

Figure 2.

Figure 2

Immunolocalization of KCNQ2 and KCNQ3 in human cortex and hippocampus. (A) Low-power view (bar equals 1 mm) of transverse section through human hippocampal formation after immunoperoxidase staining using Q2C2 antibodies and light counterstaining with hematoxylin. Subregions indicated are the dentate gyrus (DG), sections CA3 and CA1 of the hippocampus, and the subiculum (sub). Regions shown in red boxes represent locations shown at higher power in E–H. (B) Control experiment showing immunostaining was abolished by preincubation of antibodies with antigenic peptide. Adjoining sections were incubated with Q2C2 antibodies in the presence (Left) or absence (Right) of excess Q2C2 peptide. (Left) Only purple–blue counterstain of neurons and glia. (Right) Brown immunoperoxidase staining of neuronal somata, proximal dendrites, and lighter neuropil staining. (C) Confocal images of Q2C2 (Left) and Q3C1 (Right) staining of temporal cortex (parahippocampal gyrus) neurons. Red shows channel antibody staining in a punctate somotodendritic distribution; green shows brain autofluorescence, mostly associated with lipofuscin in perinuclear vesicular structures. (Scale bar equals 10 μm.) (D) Q2C2 staining of mossy fiber bundles in the dentate hilar region. (E) Q2C2 (Left) and Q3C1 (Right) staining of polymorphic neurons in the dentate hilar region. (Arrows indicate stained neuronal somata; asterisks indicate neuropil, which is stained more heavily with Q2C2 than Q3C1.) (F) Q2C2 (Left) and Q3C1 (Right) staining of neurons in the CA3 pyramidal cell layer. (G) Q2C2 (Left) and Q3C1 (Right) staining of pyramidal cells in the subiculum. (H) Staining of the neuropil in the dentate hilar and inner molecular layers by Q2C2 but not Q3C1. Arrows indicate hilar neuronal somata, labeled by both antibodies; brackets indicate inner molecular layer staining by Q2C2 but not Q3C1. (Scale bar equals 100 μm.) Pm, polymorphic layer; gc, granule cell layer; iml, inner (associational-commissural) molecular layer; oml, outer (perforant path) molecular layer. (Scale bar in B equals 20 μm in B, D–G.)