KCNQ3, cytoskeletal proteins, and PKA are associated with KCNQ2 in a Triton X-100 insoluble complex. (A) KCNQ2-associated proteins were affinity-purified using Q2C1 or Q2C2 antibodies, and probed with the indicated antibodies. KCNQ2, KCNQ3, and tubulin were detected, but PSD-95, although abundant in the starting membranes, was absent in the affinity-purified fraction. (B) AKAP79, PKA RIIB, tubulin, and actin are present in the Q2C2 affinity-purified complex, but calcineurin, CaM Kinase, NMDA-R1, and NMDA-R2 are not present.