Figure 4.

MS and Western blot analysis of leptomeningeal ADan. (A) MS analysis of the leptomeningeal amyloid isolated from the Danish case. * indicates formylated species (+28 units of mass), and @ indicates species with pyroglutamate at the amino terminus (−18 units of mass). The sequence of the full-length amyloid (34 amino acids) is indicated in one-letter code. (B) Western blotting analysis of purified amyloid fibrils from leptomeningeal vessels of cases with FDD (lanes 1 and 4) and FBD (lanes 2 and 3) reacted against anti-ADan antibody (5282) (lanes 1 and 2) and anti-ABri antibody (338) (lanes 3 and 4). Molecular masses are in kDa.