Figure 2.

DAAOX does not affect either basal or NMDA-evoked NOS activity monitored in mature cerebellum. (A) d-Serine and glycine levels were determined by HPLC in cerebellar slices from immature (9- to 11-day-old) and adult (6-week-old) rats. †, P < 0.0001; t test. (B) Basal and NMDA-evoked NOS activity was determined as in Fig. 1. Data are the means ± SEM of four independent experiments, with triplicate determinations in each case. Statistical comparisons are with respect to the corresponding controls in the absence of NMDA and DAAOX. *, P < 0.001; one-way ANOVA with Tukey's multiple comparison test. NMDA-evoked NOS activity in adult was significantly less than in the immature cerebellum (#, P < 0.05).