Figure 2.

AtNramp genes complement a yeast mutant deficient in Mn uptake. Growth of smf1 yeast cells expressing AtNramp1, AtNramp3, or AtNramp4 on SD plates supplemented (Right) or not (Left) with 20 mM EGTA. smf1 yeast cells were transformed either with empty pDR195 or with pDR195 containing the cDNAs of AtNramp1, AtNramp3, AtNramp4 or of EIN2. Transformed strains were grown overnight in liquid synthetic dextrose-ura. The cultures were diluted to an OD of 0.1 and spotted on plates containing synthetic dextrose-ura (pH 6). The plates were incubated at 30°C for 3 days before photography.