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. 1999 Oct 12;96(21):11723–11728. doi: 10.1073/pnas.96.21.11723

Figure 4.

Figure 4

Mammalian antibody-antigen two-hybrid CAT assay. CAT reporter clone (pE5C-CAT) was transfected into CHO cells with combinations of pM1 or pNLVP16 derivatives and CAT activity was scored by TLC (a) or PhosphoImager analysis (b). Two independent transfections were performed for each vector combination. Each transfection had pE5C-CAT reporter together with: lane 1, pM-β-gal + pNLscFvR4-VP16; lane 2, pE5C-CAT reporter alone; lane 3, pM-β-gal; lane 4, pNLscFvR4-VP16; lane 5:,pMβ-gal + pNLscFvF8-VP16; lane 6, pNLscFv-VP16 + pM1-AMCV; lane 7, pM-scFvR4 + pNLVP16-β-gal; lane 8, pM-scFvR4; lane 9, pNLVP16-β-gal; lane 10, pM-β-gal + pNLVP16-scFvR4; and lane 11, pNLVP16-scFvR4.