Table 1.

Geneotype of the plasmids containing DEBS genes

Vector:	pKOS021	pKOS025	pKOS010
Gene/protein:	eryAI(DEBS1)	eryAII(DEBS2)	eryAIII(DEBS3)
Module:	1    2	3	5    6
	1. Wild type
	2. AT → rapAT2
	3. KR → rapDH/KR4
	4. KR → rapDH/ER/KR1
	5. KS1°*
		6. Wild type
		7. AT3 → rapAT2
			8. Wild type
			9. AT → rapAT2
			10. KR → AT/ACP linker
			11. KR → rapDH/KR4
			12. KR → rapDH/ER/KR1
			13. Module 5 + TE†
			14. AT → rapAT2
			15. KR → AT/ACP linker
			16. KR → rapDH/KR4

The plasmids with mutant DEBS genes were constructed by cloning the specified DNA segments into one of the three vectors shown in Fig. 2. Arrows signify that the wild-type domain was replaced with the one indicated. 

*The Cys-729 → Ala null allele was created by site-specific mutagenesis. 

^†Module 6 was deleted to fuse its TE to the ACP of module 5.